Use 25 gauge 5/8 inch needle on 5cc syringe.
Pull back with some force, don't put air in the eye
Look for some tissue, if vitreous comes, eye may flatten
Can fill the eye with some saline and 30g if it flattens
Remove tenons over injection site to prevent trapping cells
Wash eye with saline after pulling out tissue
Ensure the tumor cells are in transport media- small white floaters
Place on Path specimen report that eye was punctured for cell culture
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